Table 1. sgRNA and HR templates used in this study.
All sgRNA sequences used were obtained using CRISPR4P ( Rodríguez-López et al., 2016).
| Name | Sequence |
|---|---|
| ade6 - sgRNA | TTGATAGCAACAGTGGCGAC |
| ura4 - sgRNA | CCTTGTATAATACCCTCGCC |
| meu27 - sgRNA | TATTAGCCTTTGAAGGATTT |
| clr5 - sgRNA | AGCTTGTGGCTGACCGTTAA |
| cup1:4xtetO - sgRNA | ATTTCTTTTGCTTTACGGTC |
| cup1-GFP - sgRNA | GCTCAGGCTAAACGTCGGAA |
| epe1 - sgRNA | GGACTTTTAAGATGGATTCC |
| ade6 - HR template | GCAATGACACCTCTTCCAGTAATCGGCGTTCCTGTAAAAGGAAGCACTCTTGACGGAGTTGACTCTCTTTAGTCTATTGTTCA
GATGCCTCGAGGTGTCTAGGTCGCCACTGTTGCTATCAATAATAGCCAAAATGCCGGTATTTTAGCCTGTCGTATACTTGCTACA TTTCAACCCTCC |
| ura4 - HR template | TTGGAAGACATTTCAGCCAAAAGCAAGAGACCACGTCCCAAAGGTAAACCAACTTCTTTGAGGCCTTGTATAATACCCTCGCCC
TACACTGTATGGCAATTTGTGATATGAGCCCAAGACTAAATTTTGTACACACCAGATGCATATTGTAGCTTGACGGTATTTCCAAT GTCTGCGAAT |
| meu27 - HR template | GCCAAAATCAATAGAGAACAATTATACTTTAAAAAAAAAAAATGAAGAAGGCTTCTTAAGTCAACAGGAAAATAAGTATTCAAATC
AAAATCCTTCAAAGGCTAATAACCTTTTCGACAAGCTCGGCGTAAGCAAGCCAATAACATTCATAGCTTGTATACTGCAATTGGGC GGCACCTC |
| clr5 - HR template | CTTATTTGCAGCAGCCTTTCCAAATACCCTCTCAACGTTTCTCTCGACAGCAACAATCTCATCCATTCCCTGCTGCTCAACATGCA
GTTAACGGTTAGCCACAAGCTTTGTATCCTTTCATCTACCAATCTAGAAATGTCCCAATGGGCTCCACCATGTTTGCTTCTTCAAA CCAATCTG |
|
cup1:4xtetO - HR
template |
TTGAATTAATTCATAGAGTATGATAAAAATTGATAGTAAATTCATTGGTATACTAAAGTGATGTAGAAAATTAAGAAATCACATAGAC
TACTTGAGTTACGATGATTTATTAGCATGCATcaaggcctactagtgcatgcatcgatagatcctctatcactgatagggagatctccctatcagtgata gagaggatctccctatcagtgatagagaggatctccctatcagtgatagagaggatctccctatcagtgatagagaggatcctctctatcactgataggg agatcttcaacttggtggtgaggtaaacgaaatccagTAAATATTTATGAAAAAAAAAATAAATGATTCATAACAAGCAGATGAAAATGATGA CGAATTAGGACTCTTCAAAATAAATGAAGATTATACATTACAAA |
| cup1-GFP - HR
template |
ATGACGAATTAGGACTCTTCAAAATAAATGAAGATTATACATTACAAACTTTGGTCTGACTTTTTAAAGCACACGATTTGctatttgta
tagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtggtctctcttttcgttgggatctttcgaaagggcagattgt gtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgt tgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaag aatgtttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccg tcatctttgaaaaatatagttctttcctgtacataaccttcgggcatggcactcttgaaaaagtcatgccgtttcatatgatctgggtatcttgaaaagc attgaacaccataagtgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgc atcaccttcaccctctccactgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcct ttactCCTTTTTTTGACTGACTTTTTAAGAACCTTTCCGACGTTTAGCCTGAGCTGAATGTCTGACAAAGAGTCCCACGATACAA |
| epe1Δ - HR template | GTGAACTACTCAAGAATCATAAGCACGTGGGGATAAATATTCAATGGTAGCCGAAGGAAATAAAAAGTGCCGAGGTACTTCTTA
AAAGTCCCAAAAATTA |
|
3xFLAG-epe1 - HR
template |
GAATATCAATGTCTTGATTTATAATGTCATCGTATTCAAGCCAGGAATCGCTGCCTCCTCCCTTGTCATCGTCATCCTTGTAGTCG
ATGTCATGATCTTTATAATCACCGTCATGGTCTTTGTAGTCCATCTTAAAAGTCCCAAAAATTAATTGCTTACTAGCAAAAAGGTAA CTATAAA |