Table 2. Oligonucleotides used in this study.
| Name | Sequence |
|---|---|
| Making ade6 – sgRNA - F | CtagaGGTCTCgGACTTTGATAGCAACAGTGGCGACGTTTcGAGACCcttCC |
| Making ade6 – sgRNA - R | GGaagGGTCTCgAAACGTCGCCACTGTTGCTATCAAAGTCcGAGACCtctaG |
| Making ura4 – sgRNA - F | CtagaGGTCTCgGACTCCTTGTATAATACCCTCGCCGTTTcGAGACCcttCC |
| Making ura4 – sgRNA - R | GGaagGGTCTCgAAACGGCGAGGGTATTATACAAGGAGTCcGAGACCtctaG |
| Making meu27 - sgRNA - F | CtagaGGTCTCgGACTTATTAGCCTTTGAAGGATTTGTTTcGAGACCcttCC |
| Making meu27 - sgRNA - R | GGaagGGTCTCgAAACAAATCCTTCAAAGGCTAATAAGTCcGAGACCtctaG |
| Making clr5 – sgRNA - F | CtagaGGTCTCgGACTAGCTTGTGGCTGACCGTTAAGTTTcGAGACCcttCC |
| Making clr5 – sgRNA - R | GGaagGGTCTCgAAACTTAACGGTCAGCCACAAGCTAGTCcGAGACCtctaG |
| Making cup1:4xtetO – sgRNA - F | CtagaGGTCTCgGACTATTTCTTTTGCTTTACGGTCGTTTcGAGACCcttCC |
| Making cup1:4xtetO – sgRNA - R | GGaagGGTCTCgAAACGACCGTAAAGCAAAAGAAATAGTCcGAGACCtctaG |
| Making cup1-GFP – sgRNA - F | CTAGAGGTCTCGGACTGCTCAGGCTAAACGTCGGAAGTTTCGAGACCCTTCC |
| Making cup1-GFP – sgRNA - R | GGAAGGGTCTCGAAACTTCCGACGTTTAGCCTGAGCAGTCCGAGACCTCTAG |
| Making epe1 – sgRNA - F | CtagaGGTCTCgGACTGGACTTTTAAGATGGATTCCGTTTcGAGACCcttCC |
| Making epe1 – sgRNA - R | GGaagGGTCTCgAAACGGAATCCATCTTAAAAGTCCAGTCcGAGACCtctaG |
| Making ade6 - HR template - F | GCAATGACACCTCTTCCAGTAATCGGCG TTCCTGTAAAAGGAAGCACTCTTGACGG AGTTGACTCTCTTTA
GTCTATTGTTCAGA TGCCTCGAGGTGTCT |
| Making ade6 - HR template - R | GGAGGGTTGAAATGTAGCAAGTATACGA CAGGCTAAAATACCGGCATTTTGGCTATT TATTGATAGCAACA
GTGGCGACCTAGACA CCTCGAGGCATCTGA |
| Making ura4 - HR template - F | TTGGAAGACATTTCAGCCAAAAGCAAGA GACCACGTCCCAAAGGTAAACCAACTTC TTTGAGGCCTTGTAT
AATACCCTCGCCCT ACACTGTATGGCAAT |
| Making ura4 - HR template - R | ATTCGCAGACATTGGAAATACCGTCAAG CTACAATATGCATCTGGTGTGTACAAAAT TTAGTCTTGGGCTCA
TATCACAAATTGCC ATACAGTGTAGGGC |
| Making meu27 - HR template - F | GCCAAAATCAATAGAGAACAATTATACTTTAAAAAAAAAAAATGAAGAAGGCTTCTTAAGTCAACAGGAAAAT
AAGTATTCAAATCAAAATCCTTCAAAG |
| Making meu27 - HR template - R | GAGGTGCCGCCCAATTGCAGTATACAAGCTATGAATGTTATTGGCTTGCTTACGCCGAGCTTGTCGAAAAGG
TTATTAGCCTTTGAAGGATTTTGATTTG |
| Making clr5 - HR template - F | CTTATTTGCAGCAGCCTTTCCAAATACCCTCTCAACGTTTCTCTCGACAGCAACAATCTCATCCATTCCCTGCT
GCTCAACATGCAGTTAACGGTTAGCC |
| Making clr5 - HR template - R | CAGATTGGTTTGAAGAAGCAAACATGGTGGAGCCCATTGGGACATTTCTAGATTGGTAGATGAAAGGATAC
AAAGCTTGTGGCTAACCGTTAACTGCATG |
| Making
cup1:4xtetO - HR template
- 1 - F |
TTGAATTAATTCATAGAGTATGATAAAAATTGATAGTAAATTCATTGG |
| Making
cup1:4xtetO - HR template
- 1 - R |
cactagtaggccttgATGCATGCTAATAAATCATCGTAACTCAAGTAG |
| Making
cup1:4xtetO - HR template
- 2 - F |
TTTATTAGCATGCATcaaggcctactagtgcatgca |
| Making
cup1:4xtetO - HR template
- 2 - R |
TTTTTTTTTTCATAAATATTTActggatttcgtttacctcaccacc |
| Making
cup1:4xtetO - HR template
- 3 - F |
tggtgaggtaaacgaaatccagTAAATATTTATGAAAAAAAAAATAAATGATTCATAACAAGCAGATGAAAA |
| Making
cup1:4xtetO - HR template
- 3 - R |
TTTGTAATGTATAATCTTCATTTATTTTGAAGAGTCCTAATTCGT |
| Making cup1-GFP - HR template - F | ATGACGAATTAGGACTCTTCAAAATAAATGAAGATTATACATTACAAACTTTGGTCTGACTTTTTAAAGCACAC
GATTTGCTATTTGTATAGTTCATCCA |
| Making cup1-GFP - HR template - R | TTGTATCGTGGGACTCTTTGTCAGACATTCAGCTCAGGCTAAACGTCGGAAAAGTTCTTAAAAAGTCAGTCA
AAAAAAGGAGTAAAGGAGAAGAACTTTT |
| Making epe1Δ - HR template - F | GTGAACTACTCAAGAATCATAAGCACGTGGGGATAAATATTCAATGGTAGCCGAAGGAAATAAAAAGTGCCG
AGGTACTTCTTAAAAGTCCCAAAAATTA |
| Making epe1Δ - HR template - R | CCATAGAATCTCCTTAGTTTGCATCGCAATTTTATAGTTACCTTTTTGCTAGTAAGCAATTAATTTTTGGGACTT
TTAAGAAGTACCTCGGCACTTTTTA |
| Making 3xFLAG-epe1 - HR template - F | TTTATAGTTACCTTTTTGCTAGTAAGCAATTAATTTTTGGGACTTTTAAGATGGACTACAAAGACCATGACGGT
GATTATAAAGATCATGACATCGACTA |
| Making 3xFLAG-epe1 - HR template - R | GAATATCAATGTCTTGATTTATAATGTCATCGTATTCAAGCCAGGAATCGCTGCCTCCTCCCTTGTCATCGTCAT
CCTTGTAGTCGATGTCATGATCTTT |
| Checking mutations ade6 - F | TTGTTTCAGCTCACCGCACA |
| Checking mutations ade6 - R | AAAGCAAGCAAAATCATTTAACAGT |
| Checking mutations ura4 - F | GCTCCATAGACTCCACGACC |
| Checking mutations ura4 - R | TTGTCAGTCGCGGTCGATTT |
| Checking mutations meu27 - F | AAATTTGCGCTCCTCTCTGC |
| Checking mutations meu27 - R | GTTTGGTATTTACGAGCTGCCA |
| Checking mutations clr5 - F | CACACAATGCGCACTCTTCT |
| Checking mutations clr5 - R | ACAGCAGTTGGTCCGTTAGA |
| Checking cup1:4xtetO – F (A1) | GGTTAGGCAGAAGACTTGAGCA |
| Checking cup1:4xtetO – R (A2) | ATCATCACTTGCATTCACTTCTCT |
| Checking cup1-GFP - F | GGCGAAGCTTTTAAGTCTGAAGG |
| Checking cup1-GFP - R | GCTGTCCCACTCTTACCACA |
| Checking epe1Δ – F (C1) | CAAATCTAACGAGTTTGCCTGC |
| Checking epe1Δ – R (C3) | GCAAACAACGAGTCAAAGTGGA |
| Checking epe1 cds – F (C2) | GGGCGAGCGGACAATCATAA |
| Checking 3xFLAG-epe1 - F | AGTGAGGCTGTGCAAAGGAA |
| Checking 3xFLAG-epe1 - R | TCTAACGAGTTTGCCTGCTT |
| M13F | GTAAAACGACGGCCAGT |