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. 2020 Nov 20;58(1):33–44. doi: 10.3892/ijo.2020.5150

Figure 6.

Figure 6

MYB is involved in the MAFG-AS1-mediated regulation of cell biological functions. (A) MTS was used to detect the proliferation in each group of cells. (B) The cell cycle was examined using FACS analysis. (C) RT-qPCR was used to detect the expression of the cell cycle-related genes cyclin A1 and CDK2. (D) RT-qPCR was used to detect the expression of the epithelial-mesenchymal transition-related genes E-cadherin and N-cadherin. (E) Western blotting analysis was used to detect the protein levels of E-cadherin and N-cadherin. (F and G) Annexin V-FITC was used to detect the apoptotic rates. (H) RT-qPCR was used to detect the mRNA expression levels of Bcl-2 and Bax. (I) Western blotting analysis was used to detect the protein expression levels of Bcl-2 and Bax. #P<0.05 vs. pcDNA; ФP<0.05 vs. pcDNA-shMAFG-AS1. MAFG-AS1, MAF BZIP transcription factor G antisense RNA 1; RT-qPCR, reverse transcription-quantitative PCR; sh, short hairpin RNA.