Figure 3.

Metabolic Reprogramming upon CD8⁺ T Cell Activation Requires Cardiolipin, but Not Its De Novo Synthesis

(A) Schematic of CD8⁺ T cells activated with αCD3/αCD28 +IL-2. At indicated time points cell pellets were collected for lipidomics analysis.

(B) Total CL amount in CD8⁺ T cells treated as in (A)

(C) Cardiolipin species in CD8⁺ T cells treated as in (A).

(D) Schematic of WT CD8⁺ T cells activated with αCD3/αCD28 +IL-2 with ± AD.

(E) Cytokine production of WT CD8⁺ T cells activated as in (D) analyzed at indicated time points.

(F) Schematic of WT and Ptpmt1^−/− CD8⁺ T cells activated with αCD3/αCD28 + IL-2.

(G) Cytokine production of WT and Ptpmt1^−/− CD8⁺ T cells activated as in (F) analyzed at indicated time points.

(H) WT CD8⁺ T cells activated with αCD3/αCD28 + IL-2 in a medium containing 10mM ¹³C-glucose ± AD. Twenty-four h after activation cell pellets were collected for metabolomics analysis.

(I) WT and Ptpmt1^−/− CD8⁺ T cells activated with αCD3/αCD28 + IL-2 in a medium containing 10mM ¹³C-glucose. Twenty-four h after activation cell pellets were collected for metabolomics analysis.

(J) ¹³C-glucose derived carbons incorporated into indicated metabolites in WT CD8⁺ T cells treated as in (H). Data represent mean ± SEM of 3 samples per genotype.

(K) ¹³C-glucose derived carbons incorporated into indicated metabolites in Ptpmt1 WT and Ptpmt1^−/− CD8⁺ T cells treated as in (I). Data represent mean ± SEM of 3 samples per genotype.

(L) Schematic of ¹³C-glucose tracing experiment results in WT CD8⁺ T cells ± AD, Ptpmt1 WT, and Ptpmt1^−/− CD8⁺ T cells.

Data shown as mean ± SEM of ≥3 independent experiments unless indicated. Statistical comparisons for 2 groups calculated by unpaired two-tailed Student’s t test, ^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001.

See also Figure S3.