Fig 7. Determination of virus titers of the vaccinated animals and challenged mice.

Groups of 3 Balb/c mice were immunized three times at two weeks intervals with 20μg of the cell culture based and the mouse brain derived vaccines. The control group of Balb/c mice (n = 3) was mock immunized with PBS. The IS Balb/c mice were challenged with 100 PPFU of CCHFV Turkey-Kelkit06 strain two weeks after the last immunization. All animals were sacrificed at 3 days post-infection. The virus was isolated from the blood (A), liver (B) and spleen (C) as described in the Methods. The standard deviations are shown as error bars. Two way ANOVA test was used to determine significant differeces between the groups in virus titers of vaccinated animals. **p<0.005, ***p<0.0005, ns: non-significant.