Table 1. Comparison of the serological responses in Balb/c mice intraperitoneally immunized with the cell culture based and the mouse brain derived vaccines against CCHFV.
Booster doses were given at 2 and 4 weeks after the first immunization, and virus-spesific antibodies in mice were assessed at 14, 28, and 42 days post-vaccination.
| Vaccine | Dose (μg) | Antibody titre (GMTa) | |||||
|---|---|---|---|---|---|---|---|
| First Immunization | Second Immunization | Third Immunization | |||||
| ELISA | *N ab | ELISA | N ab | ELISA | N ab | ||
| Cell culture based | 20 | 1333.3± S.D | **ND | 18466.7± S.D | 44± S.D | 76920± S.D | 114.3± S.D |
| 10 | 1596.2± S.D | ND | 6468± S.D | 11± S.D | 64533± S.D | 63.1± S.D | |
| 5 | 451.4± S.D | ND | 4041± S.D | ND | 44200± S.D | 23.3± S.D | |
| Mouse brain derived | 20 | 733.3± S.D | ND | 4200± S.D | 15.5± S.D | 38133± S.D | 52.1± S.D |
| 10 | 540.8± S.D | ND | 3900± S.D | 8.5± S.D | 32653± S.D | 9.7± S.D | |
| 5 | 581.2± S.D | ND | 1251± S.D | ND | 19000± S.D | ND | |
aThe end point antibody titre represented as the mean ± S.D. of ten animals.
* N ab; Neutralization antibody titre
**ND: Not determined