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. 2020 Dec 7;15(12):e0243506. doi: 10.1371/journal.pone.0243506

Fig 2. Islet viability after 7 days of tissue culture in control media (—) or media supplemented with 4 different doses of necrostatin-1 (25, 50, 100, or 200 μM of Nec-1) on day 3.

Fig 2

100 islet equivalents were incubated in Calcein AM (CalAM) and propidium iodide (PI) viability dyes for 30 minutes at room temperature and quantified on a microplate reader to analyze for live and dead cells, respectively. Islet viability was then calculated and expressed as percentage using the following equation: CalAM-positive cells/(CalAM-positive cells + PI-positive cells). n = 6 for each group. Data are expressed as median and interquartile range.