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. Author manuscript; available in PMC: 2020 Dec 7.
Published in final edited form as: Nat Protoc. 2019 Jun 7;14(7):2036–2068. doi: 10.1038/s41596-019-0172-4
Reagent Volume (µl) per reaction Final concentration
Oligo L1 (100 µM; Table 1) 6 15 µM
Mth RNA Ligase (included in the 5′ DNA Adenylation Kit) 8 10 µM
10× 5′ DNA Adenylation Reaction Buffer (from the 5′ DNA Adenylation Kit) 4 1×
ATP (1 mM; included in the 5′ DNA Adenylation Kit) 20 500 µM
RiboLock RNase inhibitor 1 1 U/µl
Nuclease-free distilled water 1