| Reagent | Volume (μl) per reaction | Final concentration |
|---|---|---|
| Nuclease-free distilled water | 924 | |
| 10× DNA Ligation Buffer (supplied with T4 DNA ligase from New England Biolabs, without PEG) | 120 | 1× |
| BSA (20 mg/ml) | 6 | 0.1 mg/ml |
| 10% (vol/vol) Triton X-100 | 120 | 1% |
| RiboLock RNase inhibitor | 6 | 0.2 U/μl |
| T4 DNA ligase (Thermo Fisher Scientific) | 24 | 0.02 U/μl |
| Total | 1200 |
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