| Reagent | Volume (μl) per reaction |
|---|---|
| Nuclease-free distilled water | 2.1 |
| 10× CutSmart Buffer (supplied with rSAP) | 1 |
| ATP (100 mM) | 0.4 |
| T4 DNA ligase (New England Biolabs, high concentration) | 1.5 |
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