. Author manuscript; available in PMC: 2020 Dec 7.

Published in final edited form as: Nat Protoc. 2019 Jun 7;14(7):2036–2068. doi: 10.1038/s41596-019-0172-4

Table 1 |.

Required oligos in GRID-seq

Oligo	Sequence (5′–3′)	Modification
L1	/5Phos/rGrUrUrGrGrArUrUrCrNrNrNrGrACACAGC/iBiodT/CACTCCACACACCGAACTCCAAC	5Phos: 5′ phosphorylation; rN: random ribonucleotide; iBiodT: biotin-modified thymidine
L2	/5Phos/GTTGGAGTTCGGTGTGTGGGAGTGAGCTGTGTC	5Phos: 5′ phosphorylation
A1	/5Phos/AGATCGGAAGAGCACACGTCT	5Phos: 5′ phosphorylation
A2	ACACTCTTTCCCTACACGACGCTCTTCCGATCTNN	N: random nucleotide
Primer1	AATGATACGGCGACCACCGAGATCTACAC/*****/ACACTCTTTCCCTACACGACGCTCTTCCGATCT	*: 5-nt index for multiplexing libraries
Primer2	CAAGCAGAAGACGGCATACGAGACGTGTGCTCTTCCGATCT