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. Author manuscript; available in PMC: 2020 Dec 7.
Published in final edited form as: Nat Protoc. 2019 Jun 7;14(7):2036–2068. doi: 10.1038/s41596-019-0172-4
Reagent Volume (μl) per reaction Final concentration
Oligo A1 (100 µM; Table 1) 10 25 µM
Oligo A2 (100 µM; Table 1) 10 25 µM
10× New England Biolabs Buffer 2 4 1×
Nuclease-free distilled water 16