Reagent | Volume (μl) per reaction | Final concentration |
---|---|---|
Nuclease-free distilled water | 261.6 | |
10× Tango Buffer (supplied with AluI) | 32 | 1× |
10% (wt/vol) SDS | 6.4 | 0.2% |
Reagent | Volume (μl) per reaction | Final concentration |
---|---|---|
Nuclease-free distilled water | 261.6 | |
10× Tango Buffer (supplied with AluI) | 32 | 1× |
10% (wt/vol) SDS | 6.4 | 0.2% |