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. Author manuscript; available in PMC: 2020 Dec 7.
Published in final edited form as: Nat Protoc. 2019 Jun 7;14(7):2036–2068. doi: 10.1038/s41596-019-0172-4
Reagent Volume (μl) per reaction Final concentration
Nuclease-free distilled water 261.6
10× Tango Buffer (supplied with AluI) 32
10% (wt/vol) SDS 6.4 0.2%