| Reagent | Volume (µl) per reaction | Final concentration |
|---|---|---|
| Nuclease-free distilled water | 352 | |
| 10× RNA Ligase Buffer (supplied with T4 RNA ligase 2, truncated KQ) |
40 | 1× |
| 10% (vol/vol) Tween 20 | 4 | 0.1% |
| RiboLock RNase inhibitor | 4 | 0.4 U/µl |
| Total | 400 |
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