| Reagent | Volume (μl) per reaction |
|---|---|
| Nuclease-free distilled water | 29 |
| 2 M KCl | 18 |
| 10 mM dNTP mix | 32 |
| 5× RT First Strand Buffer (included in the SuperScript III Reverse Transcriptase) | 28 |
| 100 mM DTT | 28 |
| SuperScript III RT (included in the SuperScript III First-Strand Synthesis System and the SuperScript III Reverse Transcriptase) | 5 |
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