| Reagent | Volume (μl) per reaction | Final concentration |
|---|---|---|
| Nuclease-free distilled water | 352 | |
| 10× DNA Ligation Buffer (supplied with T4 DNA ligase from New England Biolabs, without PEG) | 40 | 1× |
| RiboLock RNase inhibitor | 4 | 0.4 U/µl |
| 10% (vol/vol) Tween 20 | 4 | 0.1% |
| Total | 400 |
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