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. 2020 Dec 3;15:9745–9758. doi: 10.2147/IJN.S268282

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© 2020 Wu et al.

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Elevated ILC2s related cytokines and elicitation of CD4⁺T response following OVA-induced asthma. RT-PCR results of the relative expression of IL-33 (A), IL-13 (B), IL-5 (C) and IL-4 (D) in mice lung tissue. (E) ELISA results showed more OVA-IgE in the BALF of OVA treated mice than PBS treated mice. (F) The correlation of OVA-IgE and ST2 mRNA expression (P = 0.0305, r² = 0.4626). (G) The correlation of ILC2s% and ST2 mRNA expression (P = 0.0439, r² = 0.4164). (H) Representative examples of ﬂow cytometry analysis of CD4⁺T cells percentage in PBS and OVA treated mice lung. (I) Frequencies (n = 8) of percentage of CD4⁺T cells in mice lung. (J) The correlation of ILC2s% and CD4⁺T% (P = 0.0239, r² = 0.4930). Data are presented as mean ± SD, *P < 0.05; **P < 0.01 compared with control.