Figure 3.

Root Hair and IT Phenotypes of Ljrop Mutants.

(A) Light micrographs of root hairs in wild-type (Gifu) and Ljrop mutants at 2 d after germination. The numbers in the lower left corners show the number of plants with the type of root hair shown in the image out of the total number of plants observed. Bars = 100 μm.

(B) Light micrograph of M. loti R7A-induced root hair deformation in the infection zone of wild-type and Ljrop mutants at 18 h after M. loti R7A inoculation. Bars = 100 μm.

(A) and (B) Insets (bottom) are enlargements of the areas in red boxes (top).

(C) to (F) IT phenotypes of wild-type (C) and Ljrop mutants ([D] to [F]) at 5 d after inoculation with M. loti R7A/LacZ. Bars = 50 μm.

(G) Boxplot representing the number of infection events on roots of wild-type and Ljrop mutant roots at 5 d after inoculation with M. loti R7A/lacZ. The center line in the box shows the median; the box limits are the upper and lower quartiles; the whiskers represent the maximum and minimum values. Asterisks indicate a significant difference (**P < 0.01, Student’s t test, comparison between wild-type and Ljrop mutants).