Figure 4.

Disorientated IT Phenotypes of Ljrop6 Mutants and Complementation of IT Phenotype via Hairy Root Transformation of Ljrop6-1.

(A) to (E) Normal IT phenotypes of the wild-type (A) and typical misdirected infection events in all three Ljrop6 mutants in (B) to (D). Bars = 50 μm. (E) A cartoon diagram of the misdirected ITs. Misdirected indicates misdirected or looped IT in root hairs or epidermis cell.

(F) and (G) Boxplots representing the number of infection events in wild-type L. japonicus (Gifu) and Ljrop6 mutants at 5 d after inoculation with M. loti R7A/lacZ. Misdirected infection events are enlarged in the inset. Asterisks indicate a significant difference (*P < 0.05, **P < 0.01, Student’s t test, comparison between wild-type and Ljrop6 mutants).

(H) and (I) IT phenotype (H) and infection events (I) of Ljrop6-1 hairy roots expressing control plasmid (EV) and pLjROP6:LjROP6-mCherry. ITs were stained with X-Gal and scored 5 d after inoculation with M. loti R7A/lacZ. Misdirected infection events are enlarged in the inset. Asterisks indicate a significant difference (*P < 0.05, **P < 0.01, Student’s t test, comparison between EV and LjROP6). Bars = 50 μm.

For each boxplot, the center line in the box shows the median; the box limits are the upper and lower quartiles; the whiskers represent the maximum and minimum values.