Figure 4.

Downregulation of ChLAC8 by RNA Interference in Transgenic Cleome Plants.

(A) to (C) Visible phenotypes of ChLAC8 downregulated and null-segregant plants. Plants grown in the greenhouse for 2 months (A), 3 months (B), and 4 months (mature flowering plants (C).

(D) Seed coat phenotypes at 8, 12, 16, and 20 DAP.

(E) Transcript levels of ChLAC8 in the seed coats at 16 DAP.

(F) The C/G ratio in the seed coat at 20 and 24 DAP as determined by thioacidolysis.

(G) Lignin content in the seed coat at 20 and 24 DAP as determined by thioacidolysis.

Two independent T₂ transgenic lines (9 and 27) were analyzed and compared with null segregant plants. Approximately 2 mg of the dry weight of seed coats were used for thioacidolysis analysis per replicate. Data are means ± se derived from three biological replicates. Solid circles, triangles, and squares represent individual data points. *P ≤ 0.05 or **P ≤ 0.01 level indicates significant differences by unpaired two-tailed Student’s t test.