Figure 8.

Lignin Composition in Stems of Arabidopsis comt Mutant Expressing ChLAC8.

(A) ChLAC8 transcript levels in inflorescence stems as determined by qRT-PCR, expressed relative to Tubulin as a housekeeping gene.

(B) to (F) Lignin monomer thioacidolysis yields for hydroxyphenyl (B), caffeyl (C), guaiacyl (D), 5-hydroxyguaiacyl (E), and syringyl (F) units in inflorescence stems.

Values shown are percentages of total lignin units in stem tissues. Four independent homozygous T₃ transgenic lines expressing ChLAC8 (L) were analyzed and compared with two lines expressing GUS (G), all in the comt mutant background. For each biological replicate, the inflorescence stems were harvested from three plants. Data are means of three biological replicates (after averaging two analytical replicates). Error bars indicate ses. The different letters above the bars represent statistically significant differences determined by one-way ANOVA (Duncan, P ≤ 0.05) with the software SPSS Statistics (v22; IBM).