Table 1. Enzymatic Activity and Kinetic Parameters of Recombinant ChLAC8 Toward Different Monolignols.

Substrate	Vmax (pKAT mg−1)	Km (μM)	Kcat (s−1)	Kcat/Km (M−1 s−1)
Caffeyl alcohol	284.4 ± 50.4a	210.3 ± 90.6a	0.019 ± 0.003a	92.53
Coniferyl alcohol	N.D.	N.D.	N.D.	N.D.
Sinapyl alcohol	192.0 ± 12.8	60.2 ± 16.7	0.013 ± 0.001	218.0

Purified recombinant ChLAC8 (10 to 20 μg) was incubated with 25 to 800 μM substrates at 30°C for 30 min. Three enzyme assays were performed at each substrate concentration, and the V_max and K_m values were calculated by nonlinear regression analysis. The significance of difference in kinetic parameters between caffeyl alcohol and sinapyl alcohol was estimated with the unpaired two-tailed Student’s t test, and P-values < 0.05 were deemed significant. N.D., not detected.

^aSignificant difference found.