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. 2020 Sep 28;32(12):3812–3824. doi: 10.1105/tpc.20.00108

Figure 4.

Figure 4.

JANUS Mediates Pol II-Dependent PLT1 Transcription.

(A) Schematic illustration of the PLT1 genomic locus. Letters (a and b) indicate target regions in ChIP-PCR.

(B) Quantification of ChIP-PCR. Pol II enrichment at PLT1 is presented as the ratio of PLT1 (Pol II/input) to ACTIN (Pol II/input). Results are means ± se (n = 3). Asterisks indicate significant differences from the wild type (t test, P < 0.05).

(C) Representative PI-stained roots from the wild type, 35Spro:JANUS-RNAi-1, and two 35Spro:JANUS-RNAi UBQ10pro:GFP-PLT1 lines (Res-1 and Res-2). The region of the RAM is indicated by two arrowheads. Bar = 50 μm.

(D) Relative transcript abundance of PLT1 in the wild type, 35Spro:JANUS-RNAi-1, and two lines of 35Spro:JANUS-RNAi-1 UBQ10pro:GFP-PLT1 (Res-1 and Res-2). Values are means ± se (n = 3). RNAs were extracted from seedlings at 5 DAG.

(E) and (F) Length (E) and cell number (F) of the root meristem zone (MZ). Values are means ± sd (n > 17).

Different letters in (D) to (F) indicate significantly different groups (one-way ANOVA, Tukey’s multiple comparisons test, P < 0.05).