Figure 9.

GIF1 and JANUS Antagonistically Mediate the Transcription of PLT1 and Root Meristem Development.

(A) to (F) Representative CLSM images of PI-stained roots expressing the PLT1pro:CFP reporter in wild-type Col-0 (A), 35Spro:JANUS‐RNAi (RNAi-1; [B]), gif1 (C), 35Spro:JANUS‐RNAi gif1 (JANUS-RNAi gif1; [D]), UBQ10pro:GFP-GIF1 (GIF1-OE; [E]), and 35Spro:JANUS‐RNAi UBQ10pro:GFP-GIF1 (JANUS-RNAi GIF1-OE; [F]). Images are merges of the CFP channel (green) and the RFP channel (magenta). Bars = 50 μm.

(G) CFP intensity at the stem cell niche. a.u. indicates arbitrary units. Values are means ± sd (n > 20).

(H) Relative transcript abundance of PLT1. Results are means ± se (n = 3). RNAs were extracted from seedlings at 5 DAG.

(I) and (J) Length (I) and cell number (J) of the root meristem zone (MZ). Values are means ± sd (n > 16).

Different letters in (G) to (J) indicate significantly different groups (one-way ANOVA, Tukey’s multiple comparisons test, P < 0.05).

(K) A working model showing that two cargos regulated by IMB4 perform antagonistic roles in PLT1 expression and thus root meristem development.