Predicted Structure of the AtATG4a-AtATG8a Complex.
(A) 3D modeling of the AtATG4a-AtATG8a complex based on the structure of the HsAtg4B-LC3 protein complex (PDB ID: 2Z0E). The AtATG8 protein sequence (Q8LEM4 in UniProtkB) corresponds to the splice variant 1. Surface representation of the protein complex and the equivalent residues surrounding the catalytic cavity Cys170, Trp192, Asp364, and His366 in AtATG4a (red) and Phe116, Gly117, Thr120, and Ala122 in AtATG8a (blue) are shown in the structural models.
(B) Zoomed view of the putative conformation of the active site showing the spheres corresponding to the position and distance (Å) of catalytic residues Cys170, Trp192, Asp364, and His366 in AtATG4a.