Figure EV1. Diagram of tau and the antibodies used in the assays.

Tau protein domains and the fragments recognized by the combination of antibodies of the assays used in this study. We show the longest form of tau (2N4R), which comprises 441 amino acids. The two N‐terminal domains (N) are depicted in green, the two proline‐rich domains (P) in yellow and the four microtubule‐binding domains (R) in blue. The phosphorylation sites identified by the assays are also shown. Details of the assays can be found in the methods section. Abbreviations: Mid, mid‐region; N, N‐terminal; p‐tau, phosphorylated tau