Table 1.

Examples of chemokines and their impact on myoblast migratory capacity in vitro.

Chemokine	Concentration	‐fold increase in migration	Cell type	Assay	ECM component	Reference
HGF	10 ng/ml	5.43‐; 5‐	Primary myoblasts (rat; human)	Transwell	Fibronectin	Bischoff (1997), González et al (2017)
SDF‐1	10 ng/µl	2.5‐	Primary myoblasts (mouse)	Wound healing	Uncoated	Kowalski et al (2017)
TGF‐β	5 ng/ml; 20 ng/ml	4.42‐; 0.73‐	Primary myoblasts (rat); C2C12 myoblasts	Transwell; wound healing	Fibronectin; uncoated	Bischoff (1997), Leloup et al (2006)
IGF‐1	40 ng/ml; 100 ng/ml	0.66‐; 3.4‐	C2C12 myoblasts; primary myoblast (mouse)	Wound healing; transwell	Uncoated	Leloup et al (2006), Yanagiuchi et al (2009)
Insulin	15 µg/ml	0.97‐	C2C12 myoblasts	Wound healing	Uncoated	Leloup et al (2006)
FGF‐2	1 ng/ml; 10 ng/ml; 10 ngml; 100 ng/ml; 3.8–7.0 ng/ml	N/A; 7.8‐; 6.4‐ ; 3.4‐; N/A	Primary myoblasts (rat; mouse‐embryonic; mouse; mouse; human)	Transwell; Blind well chemotaxis chamber; Chemotaxis chamber; Chemotaxis chamber; Microfluidics device	Fibronectin; Uncoated; Fibronectin; Uncoated; Uncoated;	Bischoff (1997), Webb et al (1997), Neuhaus et al (2003), Yanagiuchi et al (2009), Ferreira et al (2015)
FGF‐4	10 ng/ml	6.7‐	Embryonic myoblasts (mouse)	Blind well chemotaxis chamber	Uncoated	Webb et al (1997)
FGF6	10 ng/ml	~5‐	Primary myoblasts (mouse)	Chemotaxis chamber	Fibronectin	Neuhaus et al (2003)
PDGF‐BB	50 ng/ml	3.3‐	Primary myoblasts (human)	Transwell	Uncoated	Piñol‐Jurado et al (2017)
5% Chick embryo extract	N/A	6.7‐	Primary myoblasts (rat)	Transwell	Fibronectin	Bischoff (1997)

In cases where multiple concentrations were assessed, the concentration that facilitated the greatest fold change of migration was taken.