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. 2020 Oct 30;12(12):e12291. doi: 10.15252/emmm.202012291

Figure 2. Disrupting SATB2 impaired GSC proliferation and self‐renewal.

Figure 2

  • A
    Immunoblot analysis of SATB2 expression in GSCs transduced with lentiviral‐mediated non‐targeting shRNA (shNT) or SATB2 shRNA (shSATB2).
  • B
    Cell viability of GSCs transduced with shNT or shSATB2 (n = 5).
  • C
    EdU incorporation assay of GSCs transduced with shNT or shSATB2. Scale bar: 50 μm.
  • D
    Quantification of (C) showing the percentage of EdU+ cells (n = 5).
  • E
    Tumorsphere images of GSCs transduced with shNT or shSATB2. Scale bar: 100 μm.
  • F, G
    Quantification of the diameter (F) or number (G) of tumorspheres formed by GSCs expressing shNT or shSATB2 (F: n = 9; G: n = 5).
  • H
    In vitro limiting dilution analysis of the tumorsphere formations of GSCs expressing shNT or shSATB2. Silencing SATB2 attenuated the self‐renewal capacity of GSCs.

Data information: Data are represented as mean ± SD. ****P < 0.0001, two‐way ANOVA analysis followed by Tukey’s test (B), one‐way ANOVA analysis followed by Tukey’s test (D, F, and G) or ELDA analysis for differences in stem cell frequencies (H). Exact P values are reported in Appendix Table S3.