Table 3.
Distribution of Products Created by Reaction of h5-LOX, h12-LOX, h15-LOX-1, and h15-LOX-2 with Substrates That Serve as Intermediates in the Biosynthesis of 5S,12S-diHpETE and 5S,15S-diHpETEa
| enzyme | substrate | C5 product (%) | C12 product (%) | C15 product (%) |
|---|---|---|---|---|
| h5-LOX | AAb | 100 | – | – |
| 12S-HpETE | 100 | – | – | |
| 15S-HETE | 100 | – | – | |
| 15S-HpETE | 100 | – | – | |
| h12-LOX | AA | – | 100 | – |
| 5S-HETE | – | 87 ± 4 | 13 ± 4 | |
| 5S-HpETE | – | 90 ± 3 | 10 ± 3 | |
| h15-LOX-1 | AA | – | 16 ± 5 | 84 ± 5 |
| 5S-HETE | – | 86 ± 6 | 14 ± 6 | |
| 5S-HpETE | – | 61 ± 6 | 39 ± 6 | |
| h15-LOX-2 | AA | – | – | 100 |
| 5S-HETE | – | – | 100 | |
| 5S-HpETE | – | – | 100 |
a
The product label (i.e., C5 product) refers to the carbon that is oxygenated on the substrate.
b
The epoxide product from 5S-HpETE was observed at low levels and considered a derivative of 5S-HpETE.