. Author manuscript; available in PMC: 2021 Oct 27.

Published in final edited form as: Biochemistry. 2020 Oct 13;59(42):4118–4130. doi: 10.1021/acs.biochem.0c00622

Table 4.

Biosynthetic Flux of Reactions Leading to the Production of 5S,12S-diHETE and 5S,15S-diHETE^a

enzyme	substrate	k_cat/K_M (s⁻¹ μM⁻¹)^c	5S,12S-diHETE flux	5S,15S-diHETE flux
h5-LOX	AA^b	0.35	0.35	0.35
h12-LOX	AA	5.8	5.8	–
h12-LOX	5S-HETE	0.051	0.044	0.0066
h12-LOX	5S-HpETE	0.15	0.14	0.015
h15-LOX-1	AA	2.1	0.34	1.8
h15-LOX-1	5S-HETE	0.23	0.20	0.032
h15-LOX-1	5S-HpETE	0.23	0.14	0.089
h15-LOX-2	AA	0.19	–	0.19
h15-LOX-2	5S-HETE	0.072	–	0.072
h15-LOX-2	5S-HpETE	0.035	–	0.035

Biosynthetic flux is calculated by multiplying each k_cat/K_M value (s⁻¹ μM^–1) by the percentage of total product from each reaction that serves as the intermediate in the synthesis of either 5S,12S-diHETE or 5S,15S-diHETE.

The AA flux values are also listed as they produce intermediates in the biosynthetic pathway, such as 5S-HpETE, 12S-HpETE, and 15S-HpETE.

k_cat/K_M error values are listed in Table 2.