Figure 5. Sepsis reduces the number of naive MOG-specific CD4 T cell precursors.

(A) Experimental design: C57Bl/6 mice underwent sham or CLP surgery. MOG-specific CD4 T cell precursors were enriched from the spleen 5 days after surgery via tetramer pulldown and enumerated. (B) Representative gating strategy for identifying MOG-specific CD4 T cell precursors from sham and CLP mice. Number of splenic MOG-specific CD4 T cell precursors in sham and CLP mice (B) 5 and (C) 25 days post-surgery. Frequency of antigen-experienced (CD44^hi) (E) total and (F) MOG-specific CD4 T cells. Data are combined from two independent experiments with 8–13 mice per group. *p<0.05. Error bars represent the standard error of the mean.