Table 1. Impact of age and ISRIB on mRNA expression in the hippocampus.
Inflammatory, ISR mediators and neuronal health targets were investigated by qPCR analysis of hippocampal lysates after two ISRIB injections. Columns: (i) mRNA targets (ii) Young group mean ± SEM (iii) Old group mean ± SEM (iv) Old + ISRIB group mean ± SEM (v) ANOVA F value (vi) Significant denotation between groups (vii) n/group.
| TARGET | Young | Old | Old + ISRIB | ANOVA | Btw Groups | n: Yg/Old/Old + ISRIB |
|---|---|---|---|---|---|---|
| Ccl2 | 1.0 ± 0.1 | 2.3 ± 0.4 | 1.6 ± 0.1 | F = 5.6* | Yg v Old** | 8/7/7 |
| Cd11b | 1.0 ± 0.0 | 1.0 ± 0.0 | 1.2 ± 0.0 | F = 6.6** | Yg v Old + ISRIB** Old v Old + ISRIB* |
8/7/8 |
| Il1-β | 1.1 ± 0.1 | 1.4 ± 0.1 | 1.7 ± 0.1 | F = 3.6* | Yg v Old + ISRIB* | 8/7/8 |
| Tnf α | 1.0 ± 0.1 | 1.6 ± 0.3 | 2.1 ± 0.4 | F = 2.7 | 7/7/8 | |
| Il-6 | 1.1 ± 0.2 | 1.8 ± 0.1 | 1.8 ± 0.2 | F = 4.1* | Yg v Old* | 8/7/8 |
| Il-10 | 1.0 ± 0.1 | 1.4 ± 0.2 | 1.5 ± 0.2 | F = 1.1 | 7/7/7 | |
| Irf7 | 1.0 ± 0.1 | 1.4 ± 0.1 | 1.2 ± 0.1 | F = 1.5 | 8/7/8 | |
| Ifitm3 | 1.0 ± 0.0 | 1.2 ± 0.1 | 1.0 ± 0.0 | F = 0.8 | 8/7/8 | |
| Isg15 | 1.0 ± 0.0 | 1.3 ± 0.1 | 1.2 ± 0.0 | F = 2.0 | 8/7/7 | |
| Ifi204 | 1.1 ± 0.2 | 1.4 ± 0.2 | 1.5 ± 0.1 | F = 1.2 | 6/7/7 | |
| Gbp5 | 1.0 ± 0.0 | 1.2 ± 0.1 | 1.1 ± 0.0 | F = 2.6 | 8/7/8 | |
| Oasl1 | 1.0 ± 0.0 | 1.8 ± 0.3 | 0.9 ± 0.1 | F = 4.9* | Yg v Old*; Old v Old + ISRIB* | 8/7/8 |
| Ophn1 | 1.0 ± 0.0 | 0.9 ± 0.0 | 1.1 ± 0.0 | F = 1.8 | 8/7/8 | |
| Bdnf | 1.0 ± 0.0 | 0.9 ± 0.0 | 1.0 ± 0.0 | F = 2.7 | 8/7/8 | |
| Gadd34 | 1.0 ± 0.0 | 0.8 ± 0.0 | 0.9 ± 0.0 | F = 1.8 | 7/7/8 | |
| Pkr | 1.0 ± 0.0 | 1.0 ± 0.0 | 1.1 ± 0.0 | F = 1.0 | 8/7/8 |