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. 2020 Oct 22;9:e59716. doi: 10.7554/eLife.59716

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© 2020, Luo et al

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Figure 2. — (A) The internal holder and dovetail adapter are slid onto one another with a dovetail joint. (B) The internal holder is held in place during subsequent construction using a stereotaxic arm mounted on a tabletop with a vise. The internal holder and dovetail adapter are secured together with set screws. (C) The screw holes used to attach the internal holder to the external chassis are drilled out by hand with a pin vise, and the entire assembly is rotated such that the dovetail adapter’s flat platform is facing up. (D) The probe is laid flat against the platform, with the silicon spacer facing down. (E) The probe is glued in place by dropping several small drops of thin-viscosity cyanoacrylate (CA) glue in the gaps between the probe and platform. (F) A 10 cm length of bare silver (Ag) wire is soldered to pads on the flex cable to electrically connect the probe’s ground and external reference. (G) The first part of the external chassis is screwed onto the internal holder. (H) The silver wire is threaded through a small hole in the external chassis so that it can be connected to the animal ground during surgery. Note that this panel is mirror-reversed relative to the other panels to better illustrate the silver wire. (I) The second part of the external chassis is laid on top and glued to the first part with several drops of CA glue. (J) Adhesive copper foil tape is wrapped around the ventral portion of the assembly to shield the probe and seal any gaps between the two parts of the external chassis. (K) The implant assembly, fully constructed. The silver wire is electrically connected to the copper shield with silver epoxy. Silver epoxy is also applied where the copper shield overlapped itself, to ensure the adhesive backing did not prevent electrical contact. C and B Metabond (Parkell) is applied to the portion of the assembly to which dental cement will be applied during surgery (Figure 3B), to improve adhesion. Note that, like panel H, this panel is mirror-reversed for a better view of the silver wire. (L) The face of the implant from which the probe shank protrudes is sealed with petroleum jelly. The petroleum jelly is applied in liquid drops from a cautery.