Figure 4. Evaluation of stress response markers in the absence of amino acid transporters.

(A) Immunoblots of inducible MDA-MB-468 KO cell lines upon metabolic stress. Cells were induced with doxycycline for 4 days, plated and cultured for 24 h or 72 h in the indicated media: Full = complete medium; Starv = medium without aromatic and hydrophobic amino acids; - Met = medium without methionine. (B) Immunoblot quantification. Boxplots represent values from three independent experiments. In the boxplots, centerlines mark the medians, box limits indicate the 25th and 75th percentiles, and whiskers extend to 5th and 95th percentiles.