Figure 1.

Microbiome and Lipidome Profiles in Antibiotic-Treated Mouse Feces

(A) The relative amount of 16S rRNA gene in the feces of mice treated with either low or high dose of ampicillin (Amp, 0.1 or 1 mg/mL), vancomycin (Van, 0.05 or 0.5 mg/mL), neomycin (Neo, 1 or 4 mg/mL), metronidazole (Met, 0.1 or 1 mg/mL), or their high dose mixture (Abx). The asterisk indicates the significance of the t-test (p < 0.001). Data are mean ± SEM (n = 5, except for the high dose Amp condition (n = 3)).

(B) The microbial composition in the feces of antibiotic-treated mice. Data are mean (n = 5).

(C) Principle component analysis of lipid profiles measured by targeted and untargeted lipidomics (n = 5 mice/condition).

(D) Volcano plot of lipids between Abx-treated and control mouse feces. Blue dots: unconjugated bile acids (1: MCA, 2: CA, 3: hyodeoxycholic acid (HDCA), 4: deoxycholic acid (DCA), 5: lithocholic acid (LCA), 6: isoLCA, 7: isoalloLCA). Red dots: conjugated bile acids (8: tauroMCA, 9: tauroCA). Green dots: linoleic acid metabolites (10: 10-hydroxy-cis-12-octadecenoic acid (HYA), 11: 10-hydroxyoctadecanoic acid (HYB), 12: 10-hydroxy-trans-11-octadecenoic acid (HYC), 13: 10-oxo-cis-12-octadecenoic acid (KetoA), 14: 10-oxo-octadecanoic acid (KetoB), 15: 10-oxo-trans-11-octadecenoic acid (KetoC), 16: 13-hydroxy-9-octadecenoic acid, 17: 13-oxo-9-octadecenoic acid, 18: 10,13-hydroxyoctadecanoic acid, 19: 10-hydroxy-cis-12-cis-15-octadecadienoic acid (αHYA), 20: 10-hydroxy-trans-11-cis-15-octadecadienoic acid (αHYC)). See also Figure S1 and Data S1.