Figure 3.

In vitro Screening of α-Chains TCR

Lymphocytes of intact B10.D2(R101) mice were transduced with the indicated TCRα or GFP and cultured in vitro for 72 h with unloaded antigen-presenting cells (APCs) (to assess the background proliferation) or with APC, loaded with heat-inactivated S. typhimurium virulent strain IE 147 (to assess the antigen-induced proliferation). The level of antigen-induced proliferation of non-transduced lymphocytes (NTCs) was used as the reference. Data are representative of one of 2 independent experiments and shown as mean ± SD for three technical repeats. ∗p ≤ 0.05, ∗∗p ≤ 0.01, ∗∗∗p ≤ 0.001 (unpaired Student's t-test). See also Figures S1, S2, and S5, Tables S1, and S3.