Figure 5.
Therapeutic Potential of Transduced T Cells, Adoptively Transferred to Infected B10.D2(R101) Mice
B10.D2(R101) mice were enterogastrally infected with LD27 (4.0–6.0 x 106 CFU/mouse) of the S. typhimurium strain IE 147. On Day 3 post-infection, mice were i.v. injected with either non-transduced cells (NTC) or T cells, transduced with the single salmonella-specific TCRα in the dose of 3.0 x 105 cells/mouse. Infected mice without the adoptive transfer were used as the control. The log number of bacterial colony-forming units (lgCFUs) was counted in the spleen on Day 7 post-infection.
(A and B) Adoptive transfer of individual types of T cells, transduced with either single TCRα SM1, SM16, or SM21.
(C and D) Adoptive co-transfer of individual types of T cells, transduced with the single TCRα as specified. (A and C) The lgCFU number per spleen. (B and D) The mean lgCFU number per spleen.
Data are presented as mean ± SD. One-way ANOVA, corrected with Tukey's multiple comparisons. See also Figures S1, S3, and S4.