Table 5.
Comparison of morphological properties between P10 microglia with and without surgery.
| E12 Ctrl | E12 MIA | |||||
|---|---|---|---|---|---|---|
| Surgery, P value | (−) | (+) | P value | (−) | (+) | P value |
| Soma area (μm2) | Soma area (μm2) | |||||
| Mean ± SD | 47.6 ± 9.58 | 47.9 ± 7.37 | 0.21 | 49.3 ± 8.24 | 47.3 ± 7.37 | 0.49 |
| Number of cells | 26 (from 4 mice) | 12 (from 4 mice) | 26 (from 4 mice) | 12 (from 4 mice) | ||
| Density (cells/mm2) | Density (cells/mm2) | |||||
| Mean ± SD | 562 ± 46.4 | 573 ± 27.6 | 0.57 | 571 ± 41.4 | 551 ± 52.2 | 0.39 |
| Number of fields | 8 (from 4 mice) | 8 (from 4 mice) | 8 (from 4 mice) | 8 (from 4 mice) | ||
| Total process length (μm) | Total process length (μm) | |||||
| Mean ± SD | 314 ± 102 | 300 ± 42.2 | 0.66 | 276 ± 60.4 | 298 ± 42.9 | 0.29 |
| Number of cells | 14 (from 4 mice) | 12 (from 4 mice) | 14 (from 4 mice) | 12 (from 4 mice) | ||
| Number of processes | Number of processes | |||||
| Mean ± SD | 4.93 ± 1.00 | 4.75 ± 0.45 | 0.57 | 4.50 ± 0.94 | 4.58 ± 0.51 | 0.79 |
| Number of cells | 14 (from 4 mice) | 12 (from 4 mice) | 14 (from 4 mice) | 12 (from 4 mice) | ||
| Number of branches | Number of branches | |||||
| Mean ± SD | 33.4 ± 15.8 | 31.3 ± 4.01 | 0.67 | 29.3 ± 6.07 | 29.0 ± 3.01 | 0.88 |
| Number of cells | 14 (from 4 mice) | 12 (from 4 mice) | 14 (from 4 mice) | 12 (from 4 mice) | ||
| E15 Ctrl | E15 MIA | |||||
|---|---|---|---|---|---|---|
| Surgery, P value | (−) | (+) | P value | (−) | (+) | P value |
| Soma area (μm2) | Soma area (μm2) | |||||
| Mean ± SD | 45.1 ± 6.45 | 44.5 ± 5.60 | 0.76 | 46.8 ± 8.16 | 45.4 ± 8.22 | 0.63 |
| Number of cells | 25 (from 4 mice) | 12 (from 4 mice) | 25 (from 4 mice) | 12 (from 4 mice) | ||
| Density (cells/mm2) | Density (cells/mm2) | |||||
| Mean ± SD | 601 ± 35.8 | 581 ± 36.9 | 0.30 | 581 ± 32.6 | 559 ± 43.9 | 0.27 |
| Number of fields | 8 (from 4 mice) | 8 (from 4 mice) | 8 (from 4 mice) | 8 (from 4 mice) | ||
| Total process length (μm) | Total process length (μm) | |||||
| Mean ± SD | 266 ± 14.9 | 277 ± 41.6 | 0.36 | 266 ± 40.9 | 275 ± 43.9 | 0.60 |
| Number of cells | 12 (from 4 mice) | 12 (from 4 mice) | 12 (from 4 mice) | 12 (from 4 mice) | ||
| Number of processes | Number of processes | |||||
| Mean ± SD | 4.67 ± 0.65 | 4.83 ± 0.58 | 0.51 | 4.58 ± 0.67 | 4.33 ± 0.65 | 0.36 |
| Number of cells | 12 (from 4 mice) | 12 (from 4 mice) | 12 (from 4 mice) | 12 (from 4 mice) | ||
| Number of branches | Number of branches | |||||
| Mean ± SD | 35.6 ± 5.05 | 33.0 ± 3.74 | 0.17 | 37.0 ± 6.08 |
33.8 ± 4.37 12 (from 4 mice) |
0.15 |
| Number of cells | 12 (from 4 mice) | 12 (from 4 mice) | 12 (from 4 mice) | |||