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. 2020 Dec 7;10:21364. doi: 10.1038/s41598-020-78206-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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TGF-β signaling regulates noncanonical pathways in the sclerotome. Sclerotome was treated with vehicle control or TGFβ1 for 2, 8 or 24 h. Immunoblot was used to determine activity of ERK, p38 and AKT. (B) ERK, (C) P38 and (D) AKT activity was quantified as the relative levels of the phosphoprotein over the total protein as determined from ImageJ scanned blots. α tubulin was used as a general loading control. (*) indicates significance, p < 0.05, n = 3. Immunoblots were cropped for clarity. Examples of uncropped blots are found in Supplementary Figures.