Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 7;10:21364. doi: 10.1038/s41598-020-78206-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Scx is required for the expression of Fmod and Adamtsl2. (A) C₃H₁₀T_1/2 cells were transduced with a scrambled siRNA control (Scram) or Scx specific siRNA for 24 h. Representative immunoblot showing Scx protein. α-tubulin is used as a loading control. (B) Quantification of Scx immunoblots comparing Scram transduced and Scx siRNA transduced cells. Protein levels were normalized to α tubulin and quantified using ImageJ and GraphPad Prism, n = 3, (*) indicates significance, p < 0.05. (C) Scx mRNA levels were determined by qPCR in Scram transduced and Scx siRNA transduced cells. Data was normalized to Hprt mRNA and analyzed using REST software, n = 6, (*) indicates significance, p < 0.05. (D) RNA was collected from cells that were transduced with Scram siRNA or Scx siRNA for 24 h and subsequently either left untreated (−) or treated with TGFβ1(+) for 8 h. Fmod and Adamtsl2 mRNA levels were determined by qPCR. mRNA levels were normalized to Hprt. qPCR data was analyzed by REST software, n = 6, (*) indicates significance, p < 0.05. Detailed results from qPCR REST analysis are shown in Tables S11, S12. Immunoblots were cropped for clarity. Examples of uncropped blots are found in Supplementary Figures.