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. 2020 Dec 7;11:6251. doi: 10.1038/s41467-020-20022-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The sequence alignments of NascB, Nasc_F5053, and Nas_S1868. The identical N-terminal and C-terminal parts of NascB/NasF₅₀₅₃ and Nas_F5053/Nas_S1868 are shaded in orange and light green, respectively. Residues less important are not shown and indicated by dashed lines. The four critical residues are bolded and highlighted by colors. b In vitro characterization of P450s and their mutants using cW_L-P_L as substrate. (I) Nasc_F5053; (II) Nas_S1868; (III) NasbB; (IV) Nas_F5053-Q65I; (V) Nas_F5053-A86G; (VI) Nas_F5053-Q65I-A86G; (VII) NascB; (VIII) NascB-Q65I; (IX) NascB-A86G; (X) NascB-Q65I-A86G; (XI) NascB-S1868fragment-7; (XII) NascB-Q65I-A86G-A284S-A288V; (XIII) Nasc_F5053-S284A; (XIV) Nasc_F5053-V288A; (XV) Nasc_F5053-S284A-V288A; (XVI) Nasc_F5053-A86K-V288P; (XVII) NAS-E synthetic standard; (XVIII) Nasc_F5053-Q65P-A86W-S284C.