Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 7;11:6256. doi: 10.1038/s41467-020-20062-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Representative images of PICS applied to HepG2 spheroids (×63/1.4, 170 μm × 170 μm × ~85 μm). The scattering potential, GLIM (ρ) was recovered, as in the original GLIM paper, by nonlinear filtering where the absolute value of the phase map is displayed on a log scale. PICS and DAPI insets were bilaterally filtered to improve contrast. b To compare the performance of PICS to conventional DAPI staining we constructed a semantic segmentation map by thresholding the DAPI and PICS image and calculating the dry mass within this map. When comparing total dry mass across twenty samples, we find the average percentage change between the PICS and DAPI images to be 4%.