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. 2020 Dec 7;10:21360. doi: 10.1038/s41598-020-77800-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PP VII-primed Macrophages affect the invasion of tumor cells by regulating STAT3 crosstalk. (a–e) LLCs and A549s were incubated with indicted macrophage CMs within 24 h. Invasion was qualified by transwell assays and is depicted as mean + SD of five random fields from triplicate samples (b). Representative micrographs from one experiment of two are shown (a). Scale bars denote 50 or 100 µm. ***p < 0.001. Immunoblotting of LLCs and A549s treated for 24 h as above (c,d). Indicated cells were analyzed by Immunoblot (e). Representative blot from one of two experiments is shown.