Fig. 4. UBR2 overexpression protects cells from CICD but not from apoptosis.
A HeLa cells were transfected to transiently overexpress (OE) the control vector (pcDNA3) or UBR2-His-tagged and then cells were either not treated (N.T) or treated with Actinomycin D (1 μM) for 8 h as an « apoptosis » stimulus. Cell death was measured by flow cytometry using a DAPI staining. Whole-cell lysates were analyzed for UBR2 over-expression by immunoblotting against His-tag, Hsp60 was used as a loading control. B as in A but cells were either untreated or treated with Act D (1 µM) and qVD-OPH (20 μM) for 48 h to induce CICD. C Clonogenic assay of pcDNA3 or UBR2-His-tagged overexpressing HeLa cells treated were treated with Actinomycin D (1 µM) and qVD-OPH (20 μM) for 24 h. Pictures were taken 10 days following the treatment and quantified (right panel). NT = not treated. Data are expressed as mean ± s.d (n = 3). *p < 0.05, **p < 0.01, N.S: non-significant according to a two-way ANOVA.