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. 2020 Dec 8;11(12):1041. doi: 10.1038/s41419-020-03258-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A UBR2 mRNA expression between healthy tissue (breast) and cancer tissue (invasive breast carcinoma). Data were obtained from the Oncomine database. B Immunoblots of UBR2 expression in the indicated breast cancer cell lines. Actin was used as a loading control. C MDA-MB-231 cells were transfected with the indicated siRNA and treated with Actinomycin D alone (1 μM) as an « apoptosis » stimulus or in combination with the pan-caspase inhibitor qVD-OPH (20 μM) as a « CICD » stimulus for 24 h. Cell death was measured by flow cytometry using a DAPI staining. Data are expressed as mean ± s.d (n = 3). D Real-time quantification of MDA-MB-231 cells transfected with the indicated siRNA and stained for Propidium Iodide in response to treatment with UV (40 J/m²) in combination with qVD-OPH (20 μM) as a CICD stimulus, using the Incucyte ZOOM Live-Cell Imaging system. NT = not treated. E DRUGSURV Database shows that High levels of UBR2 mRNA (n = 91) predicts a poor survival compared to low levels (n = 90) in patients suffering from breast cancer. F graphic summary of our work, see text for details. **p < 0.01, ***p < 0.001 according to a two-way ANOVA. N.S: non-significant. Data are expressed as mean ± s.d (n = 3) immunoblots and incucyte data are representative of 3 individual experiments.