Figure 5.

Dynamic properties of EcFtsZ in the absence of or in the presence of EcZipA or EcZapA measured by fluorescence and light-scattering assays. Note that these experiments used EcFtsZ, not PaFtsZ. These experiments were done in HMK buffer. (A) Kinetics of EcFtsZ pfs assembly measured by bodipy fluorescence. Assembly of 7 µM EcFtsZ alone is compared with assembly with 10 µM ZapA or ZipA. (B) Assembly of 7 µM EcFtsZ plus 10 µM EcZapA measured by bodipy fluorescence, compared to bundling measured by light scattering. (C) Exchange of subunits between EcFtsZ pfs was measured by FRET assay, for 7 µM EcFtsZ alone or mixed with 10 µM ZipA or ZapA.