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. 2020 Dec 7;220(1):e202001016. doi: 10.1083/jcb.202001016

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© 2020 Gihana et al.

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Figure S1. — Localization of GTP-Cdc42 in asynchronously dividing cells. (A) Representative time-lapse images showing the localization of the GTP-Cdc42 biosensor (Gic2-PBD-RFP) during mitosis in a wild-type cell without α factor treatment. Numbers indicate time in minutes from the initial polarization of Cdc42 in G1. Scale bar, 5 µm. (B) Timing of anaphase onset, Cdc42 redistribution, and spindle breakdown in wild-type cells without α factor synchronization (n = 100 cells from three experiments). (C) Gic2-PBD fluorescence intensity measurements over time (n = 30 cells for each time point indicated from three experiments). Average intensity from each cell compartment was normalized to the total intensity in the cell, and the average ratio from 30 cells was plotted for each time point. Error bars indicate SEM. (D) Cell volume of buds and mothers (n = 30 cells for each time point from three experiments; average ± SEM). (E) Growth rate before and after Cdc42 redistribution (n = 54 cells from three experiments; average ± SEM). Asterisks indicate statistically significant differences (****, P < 0.0001, paired t test). ns, not statistically significant.