Skip to main content
. Author manuscript; available in PMC: 2021 Oct 1.
Published in final edited form as: Exp Physiol. 2020 Sep 8;105(10):1700–1707. doi: 10.1113/EP088827

Figure 1. Experimental timeline for treatment, assessment of in vivo isometric torque, injury induction and tissue collection.

Figure 1.

Mice were randomly assigned to one of two treatment groups prior to an ovariectomy (Ovx) surgery: Placebo or 17β-estradiol (E2). In vivo isometric torque of the anterior crural muscles was then measured before (Pre), and 7, 14 and 21 d following repeated BaCl2-induced injuries. After recovery from the first or third injuries, tissues were collected and satellite cell number of injured and uninjured tibialis anterior (TA) muscles was measured using FACS.