(A) Representative tracings of maximal isometric torque by anterior crural muscles before (Pre) and 21 d following one, two or three BaCl2-induced injures. (B) Repeated in vivo isometric torque measurements were made on Ovx+E2 and Ovx+Placebo mice before injury, and at 7-d intervals after one, two and three BaCl2–induced injuries. Data points above shaded area represent adaptive strength gains. Ovx; ovariectomized, E2; 17β-estradiol. Sample size per group, n=7 mice. Values are presented as mean±SD. Post hoc results following a significant interaction between treatment and time are shown in B (p<0.05). *Significantly different from Ovx+E2 at given time point.