Fig. 4. NSD2 controls splenic germinal center response.

A) Nsd2^{loxSET/loxSET} (WT) and Mb1^creNsd2^{loxSET/loxSET} (NSD2ΔSET) mice were injected IP with sheep red blood cells and the percent of germinal center (GC) cells (GC; CD95⁺CD38^dull) and the percent of GC B cells in the light zone (LZ; CD86⁺CXCR4^dull) and dark zone (DZ; CD86⁻CXCR4⁺) of the spleen are indicated in the representative FACS plots. More than 3 independent experiments with 3 or more mice per group were performed. The percent and total number of GC B cells in Nsd2^{loxSET/loxSET} (WT, open symbols) and Mb1^creNsd2^{loxSET/loxSET} (NSD2ΔSET, red symbols) mice was plotted below. Each symbol represents one mouse (4 wild type and 6 NSD2ΔSET mice). Significance was determined by unpaired t-test *: p ≤ 0.05, **: p ≤ 0.01. B) T-independent immunization. Nsd2^{loxSET/loxSET} (WT, open symbols) and Mb1^creNsd2^{loxSET/loxSET} (KO, red symbols) mice were immunized with the T independent model antigen (NP-Ficoll) and titers of the antigen specific serum antibodies were determined by ELISA. Each symbol represents one mouse (5 wild type and 7 NSD2ΔSET mice in 2 independent experiments). C) T-dependent immunization. Nsd2^{loxSET/loxSET} (open symbols) and Mb1^creNsd2^{loxSET/loxSET} (red symbols) mice were immunized (day 0) and boosted (day 35) with the T-dependent model antigen NP₂₂-CGG and titers of the antigen specific serum antibodies were determined by ELISA. Data summarized for 5 mice in each group in 2 independent experiments. Significance was determined by unpaired t-test *: p ≤ 0.05, **: p ≤ 0.01, ***: p ≤ 0.001. The experiment was performed once with 5-8 mice per experimental group.